TriplexPredict: Triplex DNA Design and Stability Prediction Tool

Sequence Generation Parameters

1. Structure (5'→3'): Purine stem + Duplex loop (TTTA) + Pyrimidine stem + TFO loop + Pyrimidine stem.
2. Sequences containing three consecutive C-G•C⁺ triads are automatically filtered out, as consecutive cytosines may exhibit poor protonation efficiency and introduce potential prediction bias.
3. Click on any generated sequence entry, expand and view its Thermodynamic Stability Landscape and pH Stability Landscape.

TFO Loop Length (5-30)

Stem Length (8-12):

C-G-C Triplet Count (0-Stem Length)

Predicting Thermal stability (T₁/₂)

Na⁺ Concentration (0.03-0.5 M):

System pH (2-12):

Predicting pH sensibility (pH₁/₂)

Na⁺ Concentration (0.03-0.5 M):

System Temperature (5-95 °C):

Generated Results (Total: 0)

Purine Stem Duplex Loop (TTTA) Pyrimidine Stem TFO Loop Pyrimidine Stem